Recombinant Mouse IL-17A Protein

时间:2024年04月25日 来源:

Recombinant Biotinylated Human KIR2DL1 Protein,His-Avi Tag性能参数分子别名(Synonyms)CD158A;CD158Ankat1;cl-42表达区间及表达系统(Source)BiotinylatedHumanKIR2DL1ProteinisexpressedfromHEK293withHistagandAvitagattheC-Terminus.ItcontainsHis22-Arg242.[Accession|P43626]分子量大小(MolecularWeight)TheproteinhasapredictedMWof27.1kDa.Duetoglycosylation,theproteinmigratesto48-60kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.纯度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制剂(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重构方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.内分泌腺衍生的血管内皮生长因子(EG-VEGF),也称为(PK1),是分泌蛋白的原蛋白蛋白家族的成员。Recombinant Mouse IL-17A Protein

Recombinant Mouse IL-17A Protein,标准物质

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that also include MERS‑CoV and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N). The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 .The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). Before binding to the ACE-2 receptor, structural analysis of the S1 trimer shows that only one of the three RBD domains is in the "up" conformation. This is an unstable and transient state that passes between trimeric subunits but is nevertheless an exposed state to be targeted for neutralizing antibody therapy. Polyclonal antibodies to the RBD of the SARS-CoV-2 protein have been shown to inhibit interaction with the ACE-2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy.  Recombinant Mouse ROBO4 Protein,His Tag在分子生物学、生物化学或生物工程制药研究中也常作为工具酶用于重组融合蛋白的特异性断裂。

Recombinant Mouse IL-17A Protein,标准物质

N-糖苷酶 F (PNGase F)酶活定义(UnitDefinition)1个酶活力单位指在10μL的反应体系中,37℃条件下1小时从10μg变性RNaseB中除去超过95%的碳水化合物所需要的酶量。储存条件-15~-25℃保存,有效期1年。使用说明变性条件下蛋白质去糖基化1)在水中加入1μLBuffer1和目标糖蛋白(1-20μg),至终体积10μL;2)100℃温度下煮沸10min使其变性,冰上冷却,离心10秒;3)加入2μL的Buffer2、2μL的10%NP-40、6μL去离子水,总反应体积20μL;4)加入0.2~0.5μL的PNGase,轻轻混匀。在37℃孵育1-3h。非变性条件下蛋白质去糖基化1)在水中加入2μL的Buffer2和目标糖蛋白(1-20μg)至体积为20μL。2)加入0.5~1μL的PNGaseF,轻轻混匀。3)37°C孵育4-24h。注意:在变性条件下大多数底物能够更好的去糖基化,在非变性条件下可能需要增加PNGaseF的量和延长孵育时间。

Bovine Thrombin,High Specific Activity,>2000 IU/mg 牛凝血酶(高活力,>2000 IU/mg)本品有效切割质量比1:2000,通常在未做过预实验的情况下建议在1:1000质量比进行体系的优化,即1mg的目的蛋白加入2U的酶(2000U/mg),使用时可用生理盐水将酶配成100U/mL,有效消化缓冲液:20mMTris-HCl,含150mMNaCl,pH8.0。在20℃-37℃切割0.3-16h。注意:①具体反应温度可根据融合蛋白的性质而定,如需要低温保存的蛋白,可在4℃切割24h。②因为凝血酶会吸附在玻璃上,建议用塑料管分装冻存(-20℃)凝血酶溶液。注意事项1.此冻干产品稳定性好,2~8℃条件下保存3年,酶活力未有变化。室温条件下保存1年,酶活力未有变化。2.本产品作科研用途。3.为了您的安全和健康,请穿实验服并佩戴一次性手套操作。OSM与白血病抑制因子(LIF)密切相关,实际上,除了其在人类中的特定受体外,它实际上还使用了LIF受体。

Recombinant Mouse IL-17A Protein,标准物质

重组型TEV蛋白酶(rTEV)是经过基因工程改造和纯化后的重组蛋白酶,不仅保持天然TEV酶的功能活性,且在广谱的温度范围内表现出更强的稳定性和特异性。rTEV是一种用来切除融合蛋白上亲和标签的常用工具酶,具有很强的位点特异性,严格识别七氨基酸序列EXXYXQ↓(G/S),切割位点在谷氨酰胺和甘氨酸/丝氨酸之间。普遍应用的七氨基酸序列为:Glu-Asn-Leu-Tyr-Phe-Gln↓-Gly。rTEV在pH7.0,30℃时可达活性,但在pH6.0-8.5和温度4-30℃范围内皆有活性(见表1),使得反应条件的选择可根据目的蛋白的情况而灵活变动。另外rTEV切割后也能利用其N端的6×His标签,通过Ni-NTA树脂去除,以达到纯化目的蛋白的目的。产品性质来源(Source)大肠杆菌外观(Appearance)无色透明液体比活力(SpecificActivity)5U/μL活性定义(UnitDefinition)在1×rTEVBuffer(50mMTris,pH8.0,0.1mMEDTA,1mMDTT)中,30℃反应1h,剪切>85%的3μg底物所需要的酶量定义为一个活性单位。纯化(Purification)Ni柱亲和纯化纯度(Purity)≥95%(bySDS-PAGE)产品组分运输与保存方法冰袋运输。糖苷内切酶 H 是一种重组糖苷酶,能够对 N-糖蛋白中的高甘露糖和某些杂合型寡聚糖的壳二糖结构进行切割。Recombinant Human VAP-1 Protein,His Tag

SUMO蛋白酶识别完整的含有100个氨基酸的SUMO标签蛋白,并能高效地把SUMO从融合蛋白上切割下来。Recombinant Mouse IL-17A Protein

Name:AITRL,MouseSynonyms:Activation-inducedTNFRmemberLigand,TNFSF18,GITRL,TL-6Description:Activation-InducibleTNF-RelatedLigand(AITRL),alsoknownasGlucocorticoid-InducedTNF-RelatedLigand(GITRL),belongstothetumornecrosisfactorsuperfamily(TNFSF).AITRLisaTypeIIsingletransmembraneproteinandshareslowconservationwithintheextracellulardomainwithotherTNFSFmembers.AITRLisexpressedonmacrophages,immatureandmaturedendriticcellsandBcells.Itsreceptor,Activation-InducibleTNFRfamilyReceptor(AITR),isexpressedonTlymphocytes,naturalkiller(NK)cells,andantigen-presentingcells.AfterbindingbyAITRL,AITRcanbereleased.AITRactivationincreasesresistancetotumorsandviralinfectionsandisinvolvedinautoimmuneandinflammatoryprocesses.Inaddition,activatedAITRincreasesTCR-inducedTcellproliferationandcytokineproductionandrescuesTcellsandNKcellsfromapoptosis.RecombinantmouseActivation-InducibleTNF-RelatedLigand(rmAITRL)producedinE.Recombinant Mouse IL-17A Protein

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